In the study by Yueming Zhao et al. at Key Laboratory of Dairy Science, Northeast Agricultural University, Harbin China, the team developed a rapid, specific, and sensitive loop-mediated isothermal amplification technique combined with a lateral flow dipstick (LAMP-LFD) method to detect Salmonella targeting the siiA gene in powdered infant formula (PIF).
The results showed that the method of LAMP-LFD had a high diagnostic specificity of 100% for detection of Salmonella in PIF.
The results verified that the method of LAMP-LFD targeting the siiA gene is rapid, accurate, and sensitive for Salmonella detection in PIF, and that PMA shows great potential to be widely used to eliminate the amplicon contamination risk generated by the highly sensitive LAMP reaction in the detection process.