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Tagg: detection

Study: Salmonella detection in powdered dairy products using a novel molecular tool

In the study by Yueming Zhao et al. at Key Laboratory of Dairy Science,  Northeast Agricultural University, Harbin China, the team developed a rapid, specific, and sensitive loop-mediated isothermal amplification technique combined with a lateral flow dipstick (LAMP-LFD) method to detect Salmonella targeting the siiA gene in powdered infant formula (PIF).

The results showed that the method of LAMP-LFD had a high diagnostic specificity of 100% for detection of Salmonella in PIF.

The results verified that the method of LAMP-LFD targeting the siiA gene is rapid, accurate, and sensitive for Salmonella detection in PIF, and that PMA shows great potential to be widely used to eliminate the amplicon contamination risk generated by the highly sensitive LAMP reaction in the detection process.

Read the report in Journal of Dairy Science

New method for analyzing sodium in cheese

Staff at the Wisconsin Center for Dairy Research (CDR) recently validated X- ray fluorescence (XRF) spectrometry as a new method for analyzing sodium in cheese, providing manufacturers with the first quick and accurate method for directly measuring sodium in the presence of salt replacers.

The XRF technology, which is commonplace in the mining industry, had not previously been considered as a means for measuring sodium in cheese, but thanks to funding from the Innovation Center for U.S. Dairy, CDR staff were able to successfully develop and validate a method for both natural and processed cheeses. This work was recently published in the Journal of Dairy Science August edition, Volume 98, Issue 8, Pages 5040–5051, titled, “Evaluation of X- ray fluorescence spectroscopy as a method for the rapid and direct determination of sodium in cheese”.

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